Stability of erythrocyte ghosts: a gamma-ray perturbed angular correlation study.

Fate of lipid vesicles in vivo: a gamma-ray perturbed angular correlation study.

The structural integrity of unilamellar vesicles has been studied in vitro and in vivo by use of gamma-ray perturbed angular correlation techniques. These studies utilize 111In3+ weakly bound to the chelator nitrilotriacetic acid as a probe to monitor the percentage of intact vesicles. When complexes of 111In3+ and nitrilotriacetic acid is encapsulated in vesicles, 111In3+ exhibits a fast tumbl...

Stability of Polymersomes Containing in Using Perturbed Angular Correlation Spectroscopy

Perturbed angular correlation study of a haptenic molecule.

The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of (111)In is strongly perturbed when the 1-p-nitrophenyl-ethylenediaminetetraacetate chelate of (111)In(8+) is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average assoc...

Permeability Properties of Erythrocyte Ghosts

1. Erythrocyte ghosts from human blood were produced by gentle water hemolysis. The ghost-containing hemolysate (about 20 mN) was added to media of different composition (KCl, NaCl, glucose, sucrose, etc.) and varying concentration ranging from 8 to 840 mN. The volume changes of the ghost cells were followed by a light absorption method. The potassium and sodium concentrations were also analyze...

Dielectric dispersion of erythrocyte ghosts.

The underlying mechanism of the low-frequency dielectric dispersion, called the alpha dispersion, of osmotically lysed erythrocytes (erythrocyte ghosts) has remained open since its finding [H. P. Schwan and E. L. Carstensen, Science 125, 985 (1957)]. The alpha dispersion is peculiar to erythrocyte ghosts and has never been observed for intact erythrocytes. Numerical calculation based on interfa...